Freeze-pressing with the X-press is an excellent method for the release of components from organisms with a rigid cell wall, such as bacteria and plants however it is also suitable for extracting heat-labile substances from organisms without a rigid cell wall, such as animal tissue.

The following material has been successfully disrupted using the X-press technique

P=paste S=suspension T=tissue

Animal Tissues No.of pressings % of disruption State of material
Human Myeloma 1 90 T
Prostate carcinoma 1 90 T
Liver 1 90 T
Rat Liver 1 90 T
Heart 1 90 T
Stomach 1 90 T
Intestine 1 90 T
Brain 1 90 T
Skin 5 90 S
Muscle 5 95 T
Guinea Pig Skin 5 75 T
Chicken Muscle 5 75 T
Bone 4 60 T
Ox Cornea 4 95 T
Sclerotic 5 85 S
Spermatozoa 5 95 S
Water snail Foot 3 95 T
Torpedo Electric organ 5 85 T
Protozoa Astasia longa 3 100 S
Crithidia encapelti 3 100 P
Prototheca zopfil 4 99 S
Yeasts
Lipomyces sp 7 90 S
Candida sp 5 85 S
Saccharomyces pombe 5 90 S
Saccharomyces cerevisiae 3 90 P.S
Botanical material
Ragweed pollen 2 95 S
Foxtail pollen 4 65 P
Chlorella sp 3 90 S
Lamminaria sp 5 65 S
Cellulose fibre 7 70 S
Phaseolus auzeus (Mung bean) 2 100 T
Potato 2 90 T
Fungal material
Penicillium sp 4 95 S
Aspergillus niger 3 95 S
Aspergillus oryzae 3 95 S
Bacteria
Brucella sp 5 80 S
Bacillus anthracis 9 75 S
Bacillus megaterium 3 90 S
Lactobacillus bulgaricus 3 90 P.S
Clostridium chauvool 4 90 S
Clostridium welchii 3 90 S
Clostridium butyricum 3 95 S
Clostridium perfringens 5 90 S
Escherichia coli 3 95 P.S
Azotobacter vinelandii 3 95 S
Bordetella pertussis 3 99 P
Diplococcus pneumoniae 2 99 P
Leuconostoc mesenteroides 5 90 S
Mycobacterium phlei 5 75 S
Nocardia sp 4 90 S
Proteus mirabilis 3 90 P
Rhodospirillum rubum 3 99 S
Salmonella typhimurium 3 99 P
Staphylococcus albus 2 70 P
Staphylococcus aureus 3 75 S
Streptococcus pyogenes 3 75 P
Streptococcus faecalis 5 99 S
Cryptococcus terricolus 5 99 S